Effect of plating density on Ca
++ response to ligand stimulation of 5-HT
R-CHO (A-C) and 5-HT
R-CHO cells (D-F). Cells were plated at indicated densities, loaded with Calcium 4 and stimulated with 1 μM 5-HT. (A, B) Raw peak fluorescence, expressed as relative fluorescent units (RFUs). (C, D) Cell number was estimated by staining with crystal violet and fluorescence normalized to A590. (E, F) Fluorescence was normalized to baseline fluorescence of each individual well. Graph is representative of three independent experiments. n = 7-8; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. previous bar.