Knockdown of C/EBP reduced net growth of the distal, proximal, and intact neurites following axotomy. Representatives of transected axons in culture. Immediately following axotomy (t = 0), either CM, control siRNA, C/EBP siRNA #1, or C/EBP siRNA #2 was added into culture medium at a final concentration of 7 nM, immediately after neurite transection. The neurons were observed over additional 36 hours and images were taken at 10, 24 and 36 hours as indicated. Arrows denote the transection site.