Figure 3

Quantitative real-time PCR results for Pde6b and Rabac1 . The number of cycles required for detection of gene expression (right axis) of Pde6b (A) and Rabac1 (B) is indicated by blue diamonds (wt) and red triangles (rd1) at each of the four time points between P2 – P8. For both genes, significantly more cycles were required for detection in the rd1 retinas at all time points, indicating that the genes are down-regulated. The yellow bars indicate the rd1/wt ratio of expression (left axis). All data was normalized to the housekeeping gene HPRT.