Figure 5

Effect of WDR35 siRNA on bupivacaine- or H ₂ O ₂ -induced WDR35 expression, caspase-3 activity, and cell viability. Neuro2a cells were transfected with WDR35 siRNA (5 nM) for 24 h. Bupivacaine (2 mM) or H₂O₂ (0.5 mM) was then added. (A) WDR35 mRNA expression and (B) WDR35 protein expression were measured after treatment of Neuro2a cells with bupivacaine or H₂O₂ for 9h. (C) Caspase-3 activity and (D) cell viability were measured after treatment of Neuro2a cells with bupivacaine or H₂O₂ for the time indicated. *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control (not treated with siRNA, bupivacaine, or H₂O₂), ^#P < 0.05 and ^###P < 0.001 vs. treated with bupivacaine or H₂O₂ alone (n = 4-6).