Effect of WDR35 siRNA on bupivacaine- or H
-induced WDR35 expression, caspase-3 activity, and cell viability. Neuro2a cells were transfected with WDR35 siRNA (5 nM) for 24 h. Bupivacaine (2 mM) or H2O2 (0.5 mM) was then added. (A) WDR35 mRNA expression and (B) WDR35 protein expression were measured after treatment of Neuro2a cells with bupivacaine or H2O2 for 9h. (C) Caspase-3 activity and (D) cell viability were measured after treatment of Neuro2a cells with bupivacaine or H2O2 for the time indicated. *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control (not treated with siRNA, bupivacaine, or H2O2), #P < 0.05 and ###P < 0.001 vs. treated with bupivacaine or H2O2 alone (n = 4-6).