Figure 4

Effect of antioxidant and p38 MAPK inhibitor on bupivacaine- or H ₂ O ₂ -induced WDR35 expression. (A) Neuro2a cells were treated with various concentrations of EUK-8 for 1 h, followed by bupivacaine (2 mM) or H₂O₂ (0.5 mM) for 9 h (n = 6). (B) WDR35 protein expression with or without 100 μM EUK-8 was analyzed by Western blotting (n = 4). *P < 0.05 and ***P < 0.001 vs. control (not treated with bupivacaine or H₂O₂), ^#P < 0.05 and ^##P < 0.01 vs. absence of EUK-8. (C) Cells were treated with 10 μM SB202190 (SB) for 1 h, followed by bupivacaine (2 mM) or H₂O₂ (0.5 mM) for 9 h (n = 6). (D) WDR35 protein expression with or without SB202190 was analyzed by Western blotting (n = 4). *P < 0.05 and ***P < 0.001 vs. control (not treated with bupivacaine or H₂O₂), ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 vs. absence of SB202190.