Figure 9
From: Wnt3a induces exosome secretion from primary cultured rat microglia
Coomassie stained gel of proteins isolated from exosomal fractions derived from primary cortical neurons. Primary cortical neurons (2500000 cells) were treated with or without Wnt3a (10 nM) for 8 hours in SFM. Cell culture medium was collected, centrifuged at 10000xg for 10 minutes, concentrated using 3kDa centrifugal devices and exosomes were isolated at 100000xg. Proteins were separated by SDS-PAGE and visualised using coomassie blue. Western blot of Wnt3a and Alix in exosomal fractions derived from primary cortical neurons treated with and without Wnt3a in SFM for 8 hours (B).