Wnt3a co-localises with early endosomes intracellularly in microglia. N9 microglia. (50000 cells per treatment/coverslip on the day of plating) were treated with Wnt3a (10 nM) for 2 hours then fixed and stained with rabbit anti-Wnt3a (1:500: green staining) and mouse anti-Rab5 (1:500: red staining) (A). Nuclei were counterstained with Hoechst 33342. Scale bar represents 10 μm.