Figure 2

Macrophage functional activity in culture. A, Examples of fluorescence microscopy images of macrophages (immunostained with anti-Iba1 antibody; red) in WT or R192Q KI trigeminal ganglion cultures. Active Iba1 immunoreactive macrophages show inclusion of round-shaped Zy-FITC granules (green). Cell nuclei are shown in blue (DAPI). Scale bar = 10 μm. B, Images show peritoneal macrophages in culture (MФ). and macrophages/trigeminal co-cultures (WT+MФ and KI+MФ) processed like in A. Scale bar = 10 μm. C, Phagocytosis index for WT or R192Q KI cultures; n = 5 petri dish per group: * p < 0.05; ** p < 0.01. D, E, Cumulative probability plots to calculate the occurrence of granule uptake in endogenous Iba1 cells in WT (grey triangles, n = 93 cells) or R192Q KI cultures (squares, n = 64) as well as for peritoneal macrophages (MФ, black circles, n = 241), and from co-cultures (WT+MФ. inverted triangles, n = 202. and R192Q KI (KI+MФ .diamonds, n = 234).