Sustained and reduced alcohol preference induce ΔFosB in the amygdala, EW, and VTA . Percent ΔFosB positive neurons in regions of the amygdala (a.), EW (b.), and VTA (c.). d. and e. Representative images of ΔFosB/Nissl staining for regions where % ΔFosB positive cells significantly correlated with alcohol preference and consumption. Percent ΔFosB positive neurons = (# of ΔFosB positive neurons)/(# of ΔFosB positive neurons + # of Nissl positive neurons). Bonferroni post-hoc results (corrected for multiple comparisons): *= P < .05, **= P < .01 significant difference from Control group; # = P < .05, significant difference from High Conc. Group.