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Table 1 Sensitivities of primary neurons, SH-SY5Y human neuroblastomas and ESN to the seven classical BoNT serotypes

From: High yield derivation of enriched glutamatergic neurons from suspension-cultured mouse ESCs for neurotoxicology research

BoNT serotype

Target SNARE protein

Primary neurons EC50(pM)

SH-SY5Y EC50(pM)

ESNs EC50(pM)

95% confidence intervals

-

+

BoNT/A

SNAP-25

0.4A

5562

0.80

0.74

0.86

10B

BoNT/B

VAMP2

100B

41,650

22.50

12.91

39.30

BoNT/C

SNAP-25

13A

nr

14.24

9.68

20.93

Syntaxin-1

nr

nr

7.51

3.06

18.42

BoNT/D

VAMP2

nr

2560

3.09

2.49

3.83

BoNT/E

SNAP-25

36A

nr

66.73

52.12

85.44

43B

BoNT/F

VAMP2

1350B

≫300,000

157.82

133.25

186.92

BoNT/G

VAMP2

nr

nr

689.76

615.90

772.48

  1. Notes: Primary neurons’ values are compiled from two sources. Annotation by (A) represents 50% inhibition of neurotransmitter release in rat cerebellar neuron cultures exposed to BoNTs for 24 h (a 90% correlation was shown between neurotransmitter release and SNARE integrity) [29], whereas annotation by (B) represents cleavage of 50% of SNAP-25 protein in fetal mouse spinal cord cultures exposed to BoNT for 48 h [30]. SH-SY5Y neuroblastomas’ values indicate inhibition of 50% of noradrenalin release in SH-SY5Y human neuroblastomas cells after 72 h incubation [2]. ESN values represent 50% cleavage of SNARE proteins after a 24 h tonic intoxication. ESN EC50 values for BoNT/B, /C, /D, /F and /G are from this manuscript, while BoNT/A and /E are from McNutt et al. (2011). nr, not reported.