BoNT serotype | Target SNARE protein | Primary neurons EC50(pM) | SH-SY5Y EC50(pM) | ESNs EC50(pM) | 95% confidence intervals |
---|
- | + |
---|
BoNT/A | SNAP-25 | 0.4A | 5562 | 0.80 | 0.74 | 0.86 |
10B |
BoNT/B | VAMP2 | 100B | 41,650 | 22.50 | 12.91 | 39.30 |
BoNT/C | SNAP-25 | 13A | nr | 14.24 | 9.68 | 20.93 |
Syntaxin-1 | nr | nr | 7.51 | 3.06 | 18.42 |
BoNT/D | VAMP2 | nr | 2560 | 3.09 | 2.49 | 3.83 |
BoNT/E | SNAP-25 | 36A | nr | 66.73 | 52.12 | 85.44 |
43B |
BoNT/F | VAMP2 | 1350B | ≫300,000 | 157.82 | 133.25 | 186.92 |
BoNT/G | VAMP2 | nr | nr | 689.76 | 615.90 | 772.48 |
- Notes: Primary neurons’ values are compiled from two sources. Annotation by (A) represents 50% inhibition of neurotransmitter release in rat cerebellar neuron cultures exposed to BoNTs for 24 h (a 90% correlation was shown between neurotransmitter release and SNARE integrity) [29], whereas annotation by (B) represents cleavage of 50% of SNAP-25 protein in fetal mouse spinal cord cultures exposed to BoNT for 48 h [30]. SH-SY5Y neuroblastomas’ values indicate inhibition of 50% of noradrenalin release in SH-SY5Y human neuroblastomas cells after 72 h incubation [2]. ESN values represent 50% cleavage of SNARE proteins after a 24 h tonic intoxication. ESN EC50 values for BoNT/B, /C, /D, /F and /G are from this manuscript, while BoNT/A and /E are from McNutt et al. (2011). nr, not reported.