Figure 3

Overexpression of Diva/BclB decreases cell differentiation and neurite length. After transfection with either the empty vector (control) or Diva/BclB overexpressing plasmid(Diva/BclB), immunoblotting (A) both showed an increase in Diva/BclB expression without any change to NDPKB/Nm23H2 expression. (B) Overexpression of Diva (green) resulted in stronger immunostaining in the cytoplasm. There was no change in NDPKB/Nm23H2 (red) expression. In the Diva/BclB cells, Diva/BclB expression remained strong even after 24 hrs of differentiation. There was strong colocalization of both proteins in the cytoplasm and at the neurite. (C) Overexpression of Diva/BclB prevents translocation of NDPKB/Nm23H2 into the nucleus after differentiation. It also decreases the percentage of differentiated cells (D) and their average neurite length (E). An average of 150 cells per group was counted for graphical analyses, and the experiments were performed in triplicate (Bar =40μm, *p<0.05, **p<0.01).