Overexpression of NDPKB/Nm23H2 increases cell differentiation and neurite length. The PC-12 cells were harvested or fixed 24hrs post-transfection. (A) Immunoblotting showed that overexpression of NDPKB/Nm23H2 suppresses expression of Diva/BclB. (B) Immunocytochemistry staining with Diva/BclB (green) and NDPKB/Nm23H2 (red) antibodies show colocalization of both proteins in the cytoplasm. (C) The percentages of cells with NDPKB/Nm23H2 in the nucleus were greatly elevated after the protein was overexpressed. Both the percentage of differentiated cells (D) and average neurite length (E) of the differentiated cells were increased in the NDPKB/Nm23H2 overexpressing cells. An average of 150 cells per group was counted for graphical analyses, and the experiments were performed in triplicate (Bar=40μm, *p<0.05, **p<0.01, ***p<0.001).