Reciprocal expression of Diva/BclB and NDPKB/Nm23H2 proteins in differentiated PC-12 cells. (A) Real time-PCR showed decreased expression of Diva/BclB mRNA and upregulation of NDPKB/Nm23H2 and β-tubulin expressions. The data was normalized using GAPDH expression. (B) Western blotting show protein levels follow the same trend after differentiation. (C) Immunocytochemistry staining with Diva/BclB (green) and NDPKB/Nm23H2 (red) antibodies show colocalization of both proteins in the cytoplasm. (D) Nuclear staining of NDPK B was observed in differentiated cells, and the percentage of cells with NDPKB/Nm23H2 in the nucleus was quantified after counting an average of 300 cells. Bar=40μm; *p<0.05, ***p<0.001.