Table 1 Nucleotide sequences of primers used for gene expression study by real-time semiquantitative PCR assays.
From: Gender effect on neurodegeneration and myelin markers in an animal model for multiple sclerosis
Gene | Primer | Sequence |
|---|---|---|
BDNF | Forward reverse | 5'-gtgacagtattagcgagtg-3' 5'-gccttccttcgtgtaacc-3' |
GAD65 | Forward reverse | 5'-gcccgctataagatgtctc-3' 5'-aatcacactgtctgttcc-3' |
GAD67 | Forward Reverse | 5'-ggcatcttccactccttc-3' 5'-gacgactcttctcttccag-3' |
MBP | Forward reverse | 5'-catccttgactccatcgg-3' 5'-tttcatcttgggtcctctg-3' |
NGF | Forward reverse | 5'-gacgactcttctcttccag-3' 5'-cgtggctgtggtcttatctc-3' |
PDGFαR | Forward reverse | 5'- ctggtgcctgcctcctac-3' 5'- aactcgctggtcttgaacg-3' |
p75 | Forward reverse | 5'-agtggcatctctgtggac-3' 5'-ctacctcctcacgcttgg-3' |
trk-A | Forward reverse | 5'-tgaatctgtcctccaatgc-3' 5'-tgaagcgtctgtgtatgc-3' |
GAPDH | Forward reverse | 5'-ggcaagttcaatggcacagtcaag-3' 5'-acatactcagcaccagcatcacc-3' |