NGF, BDNF, p75 and trkA mRNAs expression level in the cerebellum. mRNA levels were studied by real-time PCR. Results are expressed as relative gene expression, from data obtained by using the equation 2-ΔΔCT . Results are presented as mean ± SEM. Eight animals were included in each group (male and female) at each time point. Panels A to D refers to the gender effect in control groups, panels E and H refer to the disease effect. No differences female and male healthy controls were observed in this group of transcripts (A, B). Statistical analysis (two-way ANOVA) shows that mRNA expression in EAE animals is similar in male (black circle) and female (white triangle) rats for all transcripts, whereas they differ at different time points (NGF: *p = 0.0253, F(3,53) = 3.365; p75: *** p < 0.0001 F(3,52) = 9.878; trkA: ** p = 0.0029, F(3,52) = 5.299).