PDGFαR and MBP mRNA expression level in the cerebellum. mRNA levels were studied by real-time PCR. Results are expressed as relative gene expression, from data obtained by using the equation 2-ΔΔCT . Results are presented as mean ± SEM. Eight animals were included in each group (male and female) at each time point. Panels A and B refers to the gender effect in control groups, panels C and D refers to the disease effect. No differences were observed in PDGFαR and MBP mRNA expression in female and male healthy controls (A, B), such as in PDGFαR mRNA expression in female (white triangle) and male (black circle) EAE animals at different DPI (C). On the contrary, statistical analysis (two-way ANOVA) shows that relative MBP mRNA expression in EAE animals in different in male and female rats (** p = 0.0028, F(1,52) = 9.831) and at different time points (*** p < 0.0001, F(3,52) = 25.14; Bonferroni post-test: 40 DPI, ** p < 0.01).