Table 1 Primary antibodies characteristics
Antibody/species | Supplier/code/lot | Immunogen | Dilution |
|---|---|---|---|
Rabbit polyclonal anti-Notch Homolog 1, Translocation-associated (Notch1) | Lifespan Biosciences | Synthetic peptide CQHSYSSPVDNTPSHQ N-terminal added cysteine Human Notch1 intracell. domain (aa 2488–2502) | 1:300 |
LS-C16928 | |||
Lot 6113061 | |||
Rabbit polyclonal anti-neurod (NeuroD1) | Lifespan Biosciences | Synthetic peptide DDDQKPKRRGPKKKKM Conjugated to malemide-activated KLH Human NeuroD1 (aa 76–91) | 1:100 |
LS-C9245 | |||
Lot 8030526 | |||
Mouse monoclonal anti-Neuron specific beta III Tubulin [TUJ-1] | Abcam | Rat brain microtubules (whole Protein) | 1:500 |
ab14545 | |||
Lot 543886 | |||
Rabbit polyclonal anti-Phospho-Histone H3 | Upstate | Linear peptide corresponding to human Histone H3 at Ser10. | 1:500 |
06-570 | |||
Rabbit polyclonal anti-Green fluorescent protein | Invitrogen | GFP from the jellyfish Aequorea victoria | 1:250 |
A11122 | |||
Goath polyclonal anti- Patched | Santa Cruz | Peptide mapping at the N-terminus of patched of mouse origin. | 1:50 |
sc-6149 | |||
Mouse monoclonal anti-PAX7 | Hybridoma bank | Recombinant chicken protein made E. coli (aa 352–523) | 1:15 |
PAX7 | |||
Rabbit polyclonal anti-sonic hedgehog | Santa Cruz | Rabbit polyclonal anti (aa 41–200) of shh of human origin. | 1:50 |
sc-9024 | |||
Mouse monoclonal anti-HNF3-ß | Hybridoma bank | Recombinant chicken protein made E. coli | 1:20 |
4C7 |