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Figure 5 | BMC Neuroscience

Figure 5

From: Sonic hedgehog (Shh)/Gli modulates the spatial organization of neuroepithelial cell proliferation in the developing chick optic tectum

Figure 5

Radial organization and immunocytochemical patterns at the end of DS1. A. Ectopic expression of pShhiEGFP in an electroporated OT (ED4.5); patches of positive NE cell bodies at the ventricular zone (VZ) and of postmitotic neurons at the premigratory zone (PMZ) can be observed. B. Hematoxylin-Eosin staining. Arrowheads point to mNE cells located along ventricular zone; they show PH3 nuclear labeling (C) and Notch reactive cytoplasm (D). Arrows point to neurons in the premigratory zone; they display nuclear NeuroD reactivity (E) and βIIITub reactive perikarya (F). Bar: 10 μm.

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