MiR-125b orchestrates cell proliferation, differentiation and migration in neural stem/progenitor cells by targeting Nestin

Table 1 Primers used to construct luciferase reporter plasmids of Nestin

Gene name	Primer sequence	Product size (bp)
Nestin 3’UTR	Forward/ SpeI: 5'- GGACTAGTGAAAAGACCATCTG -3'	431
(mir-125b sense)	Reverse/ SphI:5'- ACATGCATGCAGGGCGTGCTACTG -3'	431
Nestin 3’UTR	Forward/ SpeI: 5’- GCACTGCCGACTTACGGGTGCGGGGAGGGGA -3’	431
(mir-125b mutated 1 putative binding region)	Reverse/ SphI:5'- CACCCGTAAGTCGGCAGTGCCGGGCAGATGG -3'	431
Nestin 3’UTR	Forward/ SpeI: 5’- TGGCTGACAGGGCTGGCCGGCTAAGGTGAAA -3’	431
(mir-125b mutated 2 putative binding region)	Reverse/ SphI: 5'- CCGGCCAGCCCTGTCAGCCAGAAACCATATGTCAA -3'	431
ACTB 3’UTR	Forward/ SpeI: 5'- GGACTAGTGCGGACTATGACTTAGTTGC-3'	563
ACTB 3’UTR	Reverse/ SphI: 5'- ACATGCATGCAAGTCAGTGTACAGGTAAGC-3'	563

ISSN: 1471-2202