Table 1 Primers used to construct luciferase reporter plasmids of Nestin
Gene name | Primer sequence | Product size (bp) |
|---|---|---|
Nestin 3’UTR | Forward/ SpeI: 5'- GGACTAGTGAAAAGACCATCTG -3' | 431 |
(mir-125b sense) | Reverse/ SphI:5'- ACATGCATGCAGGGCGTGCTACTG -3' | |
Nestin 3’UTR | Forward/ SpeI: 5’- GCACTGCCGACTTACGGGTGCGGGGAGGGGA -3’ | 431 |
(mir-125b mutated 1 putative binding region) | Reverse/ SphI:5'- CACCCGTAAGTCGGCAGTGCCGGGCAGATGG -3' | |
Nestin 3’UTR | Forward/ SpeI: 5’- TGGCTGACAGGGCTGGCCGGCTAAGGTGAAA -3’ | 431 |
(mir-125b mutated 2 putative binding region) | Reverse/ SphI: 5'- CCGGCCAGCCCTGTCAGCCAGAAACCATATGTCAA -3' | |
ACTB 3’UTR | Forward/ SpeI: 5'- GGACTAGTGCGGACTATGACTTAGTTGC-3' | 563 |
Reverse/ SphI: 5'- ACATGCATGCAAGTCAGTGTACAGGTAAGC-3' |