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Table 1 Primers used to construct luciferase reporter plasmids of Nestin
| Gene name | Primer sequence | Product size (bp) |
|---|---|---|
| Nestin 3’UTR | Forward/ SpeI: 5'- GGACTAGTGAAAAGACCATCTG -3' | 431 |
| (mir-125b sense) | Reverse/ SphI:5'- ACATGCATGCAGGGCGTGCTACTG -3' | |
| Nestin 3’UTR | Forward/ SpeI: 5’- GCACTGCCGACTTACGGGTGCGGGGAGGGGA -3’ | 431 |
| (mir-125b mutated 1 putative binding region) | Reverse/ SphI:5'- CACCCGTAAGTCGGCAGTGCCGGGCAGATGG -3' | |
| Nestin 3’UTR | Forward/ SpeI: 5’- TGGCTGACAGGGCTGGCCGGCTAAGGTGAAA -3’ | 431 |
| (mir-125b mutated 2 putative binding region) | Reverse/ SphI: 5'- CCGGCCAGCCCTGTCAGCCAGAAACCATATGTCAA -3' | |
| ACTB 3’UTR | Forward/ SpeI: 5'- GGACTAGTGCGGACTATGACTTAGTTGC-3' | 563 |
| Reverse/ SphI: 5'- ACATGCATGCAAGTCAGTGTACAGGTAAGC-3' |
