Nestin 3’UTR
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Forward/ SpeI: 5'- GGACTAGTGAAAAGACCATCTG -3'
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431
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(mir-125b sense)
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Reverse/ SphI:5'- ACATGCATGCAGGGCGTGCTACTG -3'
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Nestin 3’UTR
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Forward/ SpeI: 5’- GCACTGCCGACTTACGGGTGCGGGGAGGGGA -3’
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431
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(mir-125b mutated 1 putative binding region)
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Reverse/ SphI:5'- CACCCGTAAGTCGGCAGTGCCGGGCAGATGG -3'
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Nestin 3’UTR
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Forward/ SpeI: 5’- TGGCTGACAGGGCTGGCCGGCTAAGGTGAAA -3’
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431
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(mir-125b mutated 2 putative binding region)
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Reverse/ SphI: 5'- CCGGCCAGCCCTGTCAGCCAGAAACCATATGTCAA -3'
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ACTB 3’UTR
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Forward/ SpeI: 5'- GGACTAGTGCGGACTATGACTTAGTTGC-3'
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563
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Reverse/ SphI: 5'- ACATGCATGCAAGTCAGTGTACAGGTAAGC-3'
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