Figure 8
MiR-125b regulates NS/PCs activities by targeting Nestin. (A) The Brdu incorpation assay indicated that overexpress Nestin may rescue the inhibitory effect on proliferation which miR-125b mimics bring about. Also, the Nestin siRNA may decrease the stimulus effect on proliferation which miR-125b inhibitors bring about. (B-F) The immunofulorescence results indicated that overexpress Nestin may rescue the stimulus effect on NS/PCs differentiation which miR-125b mimics bring about. Also, the Nestin siRNA may weaken the inhibitory effect on NS/PCs differentiation which MiR-125b inhibitors bring about. (B) Nestin, (C) Sox2, (D) Vimentin, (E) Map2, (F) Tuj1. (G) The migration assay indicated that overexpress Nestin may rescue the stimulus effect on NS/PCs migration which miR-125b mimics bring about. Also, the Nestin siRNA may weaken the inhibitory effect on NS/PCs migration which miR-125b inhibitors bring about. Bars show mean ± SD. All experiments were repeated three times. * P < 0.05 vs. ctr, ** P < 0.01 vs. ctr.