Neurones | Control | Epilepsy | Antagomir | Antagomir-control |
|---|
| Â | CA1 | CA3 | CA1 | CA3 | CA1 | CA3 | CA1 | CA3 |
|---|
Percent Death | 17.91 ± 4.78% | 20.57 ± 5.02% | 48.86 ± 12.39% a | 40.39 ± 11.42% a | 30.49 ± 8.91% b | 24.80 ± 5.80% b | 49.35 ± 12.74% | 40.69 ± 10.97% |
Percent Survival | 88.15 ± 10.02% | 78.89 ± 8.92% | 47.32 ± 11.93% a | 51.25 ± 14.80% a | 72.81 ± 10.60% b | 68.28 ± 11.54% b | 46.26 ± 9.50% | 52.05 ± 14.70% |
- Notes: In rat hippocampal CA1 and CA3 regions, the number of dead neurones or apoptotic cells was significantly increased and the number of surviving neurones was markedly decreased at 7 days post-SE, as detected by the TUNEL-assay and Nissl-staining (aP < 0.01, epilepsy vs control, n = 6 /group). In the same regions, however, neuronal death or apoptosis was decreased and neuronal survival was increased significantly after miR-34a antagomir or antagomir-control treatment (bP < 0.01, miR-34a antagomir vs antagomir-control, n = 6 /group). The survival rate is determined by the number of surviving neurones/ number of total cells × 100%, whereas the neuronal death or apoptotic rate is determined by the number of dead neurones or apoptotic cells/ number of total cells × 100%.
