Figure 2

The quantification analysis of Hes1 mRNA and Notch1 NICD protein. (A) The quantification of the real-time PCR analysis of Hes1 mRNA in the striatum of rat brain compared with GAPDH respectively in CON and EA Before I/R (lane 1, 2), CON and EA I/R2h (lane 3, 4), CON and EA I/R24h (lane 5, 6), CON and EA I/R72h (lane 7, 8) groups. (B) Representative Western blot bands of Notch1 NICD (80 kDa) expression obtained from rats in CON and EA Before I/R (lane 1, 2), CON and EA I/R2h (lane 3, 4), CON and EA I/R24h (lane 5, 6), CON and EA I/R72h (lane 7, 8) groups. (C) The bar graph shows quantification of the Western blot analysis for the Notch1 NICD protein compared with β-actin. Pretreatment with EA significantly up-regulated the Hes1 mRNA and Notch1 NICD protein expressions at 2 and 24 h after the end of ischemia/reperfusion. While the expressions of Hes1 mRNA and Notch1 NICD protein of EA group were down-regulated at 72 h compared to CON group (*P < 0.05 vs. CON of the same time point, # P < 0.05 vs. before I/R of the same group).