Figure 4

Western blot analysis of apoptosis-related proteins in the primary cortical neurons at GD 17.5. Cells were treated for 12 h with normal media as control (C), ethanol (EtOH, 100 mM), TQ (25 μM) plus ethanol (TQ+EtOH), Met (10 mM) plus ethanol (Met+EtOH), TQ plus Met plus ethanol (TQ+Met+EtOH), respectively. For proteins samples, we used the same drug treatment i.e., TQ and Met were cotreated with (100 mM) ethanol for 12 h. β-actin is the loading control in each case (A) Immunoblots of Bcl-2 and (B) Bax (C) cytochrome-c (D) cleaved caspase-9 (E) caspase-3 (F) cleaved PARP-1. Immunoblots are also shown with their respective histograms. Density values were expressed as mean ± SEM (n = 4) of the corresponding proteins and expressed as arbitrary units. Detail procedures are mentioned in materials and methods section. ^#P < 0.05 significantly different from control; *P < 0.05 different from ethanol.