Effect of Ethanol on elevation of cytosolic free [Ca2+]
in primary cortical neurons. Cells were treated for 12 h with normal media as control (C), ethanol (EtOH, 100 mM), TQ (25 μM) plus ethanol (TQ+EtOH), Met (10 mM) plus ethanol (Met+EtOH), TQ plus Met plus ethanol (TQ+Met+EtOH), respectively. TQ and Met were cotreated with (100 mM) ethanol for 12 h, which was followed by Fura-2 AM labeling. The fluorescence spectra for [Ca2+]c were measured with a luminescence spectrophotometer. Different groups are indicated with respective colors by line in the representative spectra. The uppermost Red line in the spectra indicate ethanol-elevation in [Ca2+]c level compared with the other respective control. Spectra represent means ± SEM of triplicate samples (n = 3) and represent at least one of three independent experiments.