Met and TQ prevent ethanol-induced neurotoxicity in cultured cortical neurons. (A) MTT assay of cell viability in primary fetal rat cortical neurons treated with 100 mM ethanol (EtOH) for 12 h. Cells were treated for 12 h with normal media as control (C), ethanol (EtOH, 100 mM), TQ +EtOH (TQ: 10, 15, 25 and 35 μM), TQ (25 μM) plus ethanol (TQ+EtOH), Met (10 mM) plus ethanol (Met+EtOH), TQ plus Met plus ethanol (TQ+Met+EtOH), respectively. (B) Percentage of cell viability with selected concentrations of TQ (25 μM), 10 mM Met, 100 mM EtOH; in all experiments, TQ and Met were co-incubated with ethanol for a 12 hours time period. Data are the mean ± SEM of three independent experiments (n = 3), with 3 plates in each experiment. Symbols: #P < 0.05 significantly different from control; *P < 0.05 different from ethanol.