inhibits the decrease in synaptophysin expression caused by HuPrP106-126 treatment. (A) Primary cortical neurons were either left untreated, or treated with 40 μM HuPrP106-126 for 30 minutes, 1 hour or 24 hours. Some samples were pre-treated with 1 μM PACOCF3 before addition of peptide. The cellular localization of synaptophysin was analyzed by immunofluorescence using anti-synaptophysin (green) antibody. The nuclei were stained with DAPI. Scale bar: 50 μM. (B) Intensity of synaptophysin labelling was measured and normalised to cell number. Data expressed as mean ± S.D. of three experiments, *P < 0.05.