Tubulisation and microscopic aspects of the distal segments in nerves of 0.4% agarose treated rats 70 days after. (A) Regeneration cable within the tube of a 0.4% agarose-treated rat. Agarose gel in this concentration is not a barrier to the cable growth. Left = proximal. Right = distal. Bar = 2 mm, (B) Filiform cable in a rat nerve treated with DMEM. Left = proximal. Right = distal. Bar = 2 mm, (C) Three myelinic fibres in the distal segment of a 0.4% agarose-treated rat (arrows). Semithin section (0.5 μm). Toluidine blue. Bar = 20 μm, (D) Endoneurial area of the distal sciatic nerve segment from an animal treated with 0.4% agarose. The presence of some digestion chambers filled with myelin debris indicates that Wallerian degeneration occurred (arrows). Semithin section (0.5 μm). Toluidine blue. Bar = 20 μm.