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Figure 5 | BMC Neuroscience

Figure 5

From: Comparative analysis of deutocerebral neuropils in Chilopoda (Myriapoda): implications for the evolution of the arthropod olfactory system and support for the Mandibulata concept

Figure 5

Geophilomorpha. A Geophilus carpophagus. B Single horizontal optical section (cLSM) of an autofluorescence preparation of the brain of Haplophilus subterraneus showing olfactory glomeruli (asterisks) and the structural composition of the corpus lamellosum (arrow). C 3D reconstruction of the brain of H. subterraneus with deutocerebral neuropils and midline neuropil. Blue = olfactory glomeruli, yellow = corpus lamellosum, red = midline neuropil. Contralateral connection of the CL is not shown. D Single horizontal optical sections (cLSM) of a neurobiotin backfill of the right antennal nerve in Stigmatogaster dimidiatus from ventral to dorsal. Left: several somata stained by the neurobiotin backfill (arrow) and neurite projections into a circumesophageal connective. Middle: Antennal nerves and olfactory glomeruli. Right: The slightly concave appearance of the olfactory lobe. Inset: Sagittal optical section of the same preparation showing the structural composition of the corpus lamellosum (arrow) and neurite projections (asterisk). E Single horizontal optical sections (cLSM) of an autofluorescence preparation of the brain of S. dimidiatus from dorsal to ventral. Olfactory glomeruli (asterisks) and the contralateral connection between the posteroventral OG (arrow). E' Concave appearance of the olfactory lobe (arrow). E'' ventrolateral position of the corpus lamellosum. F 3D reconstruction of the brain of S. dimidiatus with deutocerebral neuropils and midline neuropil. Blue = olfactory glomeruli, gray = bigger ventral olfactory glomerulus, yellow = corpus lamellosum, red = midline neuropil. Abbreviations: cec circumesophageal connective, cl corpus lamellosum, mn midline neuropil, na nervus antennalis, np neurite projections, ol olfactory lobe, pc protocerebrum, vog ventral olfactory glomerulus. Scalebars: A = 5 mm, B, D, E = 100 μm.

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