Figure 4
From: Calcium signals in the nucleus accumbens: Activation of astrocytes by ATP and succinate
Molecular dissection of ATP-evoked burst-like Ca2+ signalling in the NAc slice. A: Cell number-based profiling. Left - ATP-evoked of Ca2+ transients: Effects of gap-junction blockers CBX, FFA; P2 antagonists SUR and MRS2179; Na+ channel blocker TTX; Ca2+ store depletor CPA with/without added external Ca2+. Right - 2-Me-S-ADP evoked Ca2+ transients: Effect of MRS2179. B: Fluorescence intensity-based profiling. Left - ATP-evoked of Ca2+ transients: Effects of gap-junction blockers CBX, FFA; P2 antagonists SUR and MRS2179; Na+ channel blocker TTX; Ca2+ store depletor CPA with/without added external Ca2+. Right - 2-Me-S-ADP evoked Ca2+ transients: Effect of MRS2179. C: Correlation of data obtained through cell number- and fluorescence intensity-based evaluation of Ca2+ transients. The effects of tested compounds/conditions were compared to the control using Mann-Whitney with Bonferroni post hoc test (*p < 0.05 and **p < 0.01).