Effect of Asb-4 on basal and insulin stimulated phospho AKT. HEK cells were transfected with Myc-Asb-4 or empty vector. Cell lysates were blotted with antibodies as indicated. The membranes were stripped and reblotted with anti-Myc and anti-AKT antibodies, respectively (A). B. Densitometry analysis of three separate experiments (n = 3). *p < 0.05 vs. control (group 1); +p < 0.5 vs. group 3.