Figure 4

Reduced potential of TG WT and TG G93A microglia to phagocytose apoptotic neuronal cell debris. (A) Scoring scale (from 1 to 6) used to measure microglial phagocytic potential. Bar = 10 μm. (B) Representative images of NTG (G93A) and TG G93A microglia immunostained with anti-integrin β-1 antibody (green) after incubation with VybrantDiI-labelled apoptotic neuronal cell debris (red-orange). Scale bar = 50 μm. (C) Percentage of microglia with moderate to high phagocytic potential (scores 4 to 6). Data are mean ± SEM; Student's t test, *** p = 0.0001 versus respective NTG cells, n = six NTG (WT) and seven TG WT mice from three litters, and six NTG (G93A) and six TG G93A mice from two litters, capturing 10-12 images per mouse.