Figure 6

Electrophysiological assessment of GG3.1 iPSC-derived neurons. (A) Micrograph showing a representative neuronal cell that was targeted for recording. (B) Average resting membrane potentials for one set of experiments shows no discernable difference between EP and LP neurons. (C) Representative traces for whole cell-patch clamp recordings in EP, LP and WT ESCs. Action potentials were elicited with 500 ms long current injections of 2-340 pA. Current recordings were generated by stepping up membrane potential from -90 mV to +60 mV in 10 mV increments.