Neurons derived from GG3.1 iPSCs exhibit characteristic neuronal morphologies and are immuno-positive for several neural markers. (A-C) Representative images of Ni day 7 cells, fixed and stained for (A) HuC/D and Synaptophysin (Syn), (B) neural nuclei (NeuN) and Map2, or (C) Brn3a and Map2. Nuclei are stained with DAPI. (D) TuJ1 and Synaptophysin staining reveals the presence of growth cones (arrows) and presumptive synaptic boutons (star) in late-passage GG3.1 cultures on Ni day 7, indicating functional maturity. Bar indicates 50 μm.