Figure 1

iPSC lines subjected to neural induction (Ni) produce populations of neurons with similar morphology to ESC-derived neurons. (A) Ni proceeds in three stages: (1) expansion of undifferentiated cells, (2) formation of embryoid bodies (EB) in a floating serum-free culture and (3) neuronal differentiation on poly-d-lysine/laminin coated plates in the presence of N2-supplement and brain derived neurotrophic factor (BDNF). (B, C) Representative micrographs for the miPS-25 (B), GG3.1 (C) and wild-type (WT) ESC lines at critical time points of neural induction: Undifferentiated, EB day 5 and Ni days 3 and 7. Bars represent 100 μm.