CNPase status in cerebral cortex, striatum/mesencephalon, cerebellum, and hippocampus. (A-D) Densitometry analysis of immunoblots prepared from lysates of cerebral cortex, striatum/mesencephalon, cerebellum, and hippocampus of WT (white bars) and Ctsk-/- mice (grey bars) as indicated. Representative immunoblots are shown in the lower panels; lanes represent separate individuals. (A) CNPase levels showed no significant difference in cerebral cortex of Ctsk-/- mice compared to WT controls (A, Ctsk-/- n = 10; WT n = 13). (B) In striatum/mesencephalon CNPase levels were significantly lower in Ctsk-/- mice, reaching only 58% of values measured in WT (B, Ctsk-/- n = 10; WT n = 10). (C) In cerebellum CNPase levels were significantly increased in Ctsk-/- mice by 50% as compared to WT (C, Ctsk-/- n = 10; WT n = 11). (D) CNPase levels in hippocampus were significantly decreased in Ctsk-/- mice, reaching only 40% of values measured in WT controls (D, Ctsk-/- n = 11; WT n = 11). Levels of significance are denoted as * for p < 0.05; ** for p < 0.01.