Figure 3
From: Circadian rhythms in the pineal organ persist in zebrafish larvae that lack ventral brain
Pineal Exorh protein expression is indistinguishable between cyc mutant and WT embryos. Embryos were fixed and processed for whole mount antibody staining with the anti-Rhodopsin antibody 4D2. Quantification of fluorescent antibody signal shows that optical density of the pineal immunostaining is not significantly different at either time point (46 hpf, ZT 22, n = 10 embryos, p = 0.41; 72 hpf, ZT 0, n = 10 larvae, p = 0.67). Images are dorsal views with anterior to the top. Experiment was repeated two times with similar results, and representative embryos are shown. Scale bar = 20 μm.