Founctional test of the differentiated MSCs. Cholinergic neurons and dopaminergic neurons were investigated by double labeled immunofluorescence with the antibodies against ChAT (green) and NeuN as well as TH (green) and NeuN (red) with the purpose of finding founctional neurons. NeuN positive cells co-expressed with ChAT (A) or TH (B) in the CART-treated group and the bFGF/EGF-incubated group. (C) The transferred neurons exhibited cytoplasmic dark blue praticles and light blue nucleus by Nissl stain. MSCs devoid of CART displayed dark blue nucleus and light blue cytoplasm (scale bar = 50 uM).