High frequency stimulation in 10 mM Ca2+saline revealed enhanced depression in jar322/Df(3R)crb87-5 mutants. Data is shown as a percent of the average of the first 16 evoked junctional potentials (EJPs) recorded at 1 Hz. This baseline recording of 16 EJPs at 1 Hz was followed by a stimulation of 10 Hz recorded over 10 minutes and terminated with a 10 minute recording at 0.1 Hz. Following the first 25 seconds from the onset of 10 Hz stimulation, every tenth recording is shown. The initial depression in EJP amplitude measured relative to EJP amplitude at the onset of high frequency stimulation was significantly greater for jar322/Df(3R)crb87-5 larvae than the control larvae (ANOVA, p < 0.01).