jaguar loss of function mutants exhibited defects in basal synaptic transmission. Electrophysiological recordings from muscles 6/7 of third instar larvae revealed EJP amplitude was significantly reduced in the most severe jar mutant, jar322/Df(3R)crb87-5 MN, compared to the control (Dunnett's Multiple Comparison Test, *** = p < 0.001) (A). Sample traces of EJP recordings taken at 1 Hz, shown in the same order as listed for the graphs (B). mEJP frequency was significantly lower for jar322/Df(3R)crb87-5 and jar322/Df(3R)crb87-5 MN larvae compared to the control larvae (Dunnett's Multiple Comparison Test, *** = p < 0.001) (C). There was no significant difference in mEJP amplitude between jar mutants and the control (ANOVA, p > 0.05) (D). Sample traces of electrophysiological recordings from muscles 6/7 over 2 seconds, shown in the same order as listed for the graphs (E). Bars represent mean ± SEM.