From: Cyto-, myelo- and chemoarchitecture of the prefrontal cortex of the Cebus monkey
 | Nissl | WFA | SMI-32 | Myelin |
---|---|---|---|---|
10 | Well developed granular layers. Small-sized cells in layer III and more densely packed cells in Va. Vb almost blended with layer VI where small-sized neurons predominated. | Very lightly stained. Labeling concentrates in V-VI. | Moderate immunostaining. SG band with weak neuropil labeling, some fragments of apical dendrites and somas sparsely distributed. Neuropil in IG band is more densely stained and a few somas in Va. | Poor to moderate myelination, with thin vertical fibers in IG layers. |
9d | Cells in IIIc more stained and separated from layer Va by a poorly developed layer IV. Layers Vb and VI have small-sized cells and no clear limits. Vertical striations in V and IIIc. | Intensely stained band in VI and a fainter band in Va with some vertical fibers. A few nets in IIIc | Intensely stained. Many cells and processes deeply stained in SG band and a few in IG band. | Moderate to intense myelination. IG layers with vertical fibers. Evident oBb. Sparse fine fibers plexus in SG layers |
46dr | Granular. Cells are more densely packed in layer V than in III. Cellular density in layers II and IV decreases dorsally. | Moderately stained. A few small nets with poorly stained neuropil in SG layers. Staining in IG layers similar to 9d, but less intense. | Moderately stained. Clear-cut boundaries with 9d. Bilaminar aspect. Small- to medium-sized pyramidal neurons intensely stained in IIIc, and very few in V. | Lighter myelination than 9d. oBb is narrower and less stained, and vertical fibers are more sparse and thinner. |
46d | Granular. Well developed and densely packed layer II. Medium-sized cells intensely stained in Va. SG layers more prominent than IG layers. | Very lightly stained. Faintly stained band of neuropil with some dark nets in IG layers. Very few nets in III and IV. | Lightly stained. Clear limits with 46dr. Very weak neuropil staining and a few clusters of cellular bodies in III. Neuropil and dendrite fragments in IG band | Moderate myelination. Faint iBe and sparse thin vertical fibers in IG layers. Sparse plexus and horizontal fibers in SG layers. |
46v | Similar to 46d. No limits between layers V and VI. | Similar to 46d, but weaker. | Weaker than in 46d. almost no immunoreactive somas can be observed | Similar to 46d |
46vr | Granular. Layer IV more developed and densely packed than in 46v. Clear lamination in III. SG and IG layers equally prominent. Radial striations in V. | Moderately stained. Good neuropil labeling in Vb, with high concentration of nets. Labeling weaker in VI. SG layers poorly stained, with some sparse nets. | Moderately stained. Clear-cut boundaries with 46v. Immunoreactive cells in IIIb-IIIc. IG band with diffuse neuropil, neuronal processes and a few stained cells. | Moderate myelination. IG layers more heavily myelinated than 46v, with well-stained vertical fibers. oBb less evident. SG layers poorly myelinated. |
12l | Granular. Layer IV narrower than in 46vr, and lamination in III less evident. No obvious predominance between SG and IG layers. Caudally, darkly-stained cells in IIIc and Va. | Intensely stained. Denser band in VI, with neuropil and many nets reaching layer VI. Paler band in IV-III with nets and poorly stained neuropil. | Intensely stained. Many stained neurons in V-VI, but a higher number in the SG band. Bilaminar pattern. | Moderate to intense myelination. Evident oBb and heavy staining in IG layers. |
8d | Granular. In IIIc and Va cells are of medium size and darkly stained. Layers Vb and VI have cells of small size and are less stained. | Lightly stained, with a band of neuropil in IV and a few moderately stained nets in IV and III. | Moderately stained, with medium to small stained cells in IIIc, and in a lesser degree in IV, IIIa and V. | Distinct oBb and thin vertical fibers. SG layers poorly myelinated. |
8v | Similar to 8d, but cells in layer IV more densely packed. | Similar to 8d, but with darker neuropil staining in IV. | Similar to 8d but with a denser staining of neuropil. | Similar to 8d |
45 | Granular. Large-sized and darkly stained pyramidal cells In IIIc and Va. | Moderately stained. Band with dense nets in IV, reaching III and Va. Moderately stained neuropil in VI. Faint band in Vb. | Moderately stained. Bilaminar aspect. Cell body fragments and intense neuropil in SG band. Pale neuropil in IV. Moderate labeling (neuropil and fragments) in IG band. | Moderate myelination (heavy myelination in IG layers). SG layers poorly myelinated. |