Microglial Iba-1 and iron histochemical double staining in cEAE mice. (A) Reactive microglia Iba-1 staining could be present around vessels without associated iron deposits, or conversely, (B) iron staining could be present around vessels without associated microgliosis. (C) An iron labeled vessel with associated microgliosis and double labeled cells revealing iron deposits within reactive microglia (arrowheads). (D) Reactive microglial cells containing iron deposits (arrowheads). Bars = 50 μm.