Summary of the immunolocalization of the sNPF receptor in the queen brain and SEG. A total of ~164 cells (shown as red dots) distributed in cell clusters (C1-C9 and C12) or as individual cells (C10, C11) were identified. Six cell clusters (C1-C6) are seen in anterior view (A) and C7-C12 (clusters or individual cells) are seen in the posterior view (B) of the queen brain and SEG. Dorsal views (C and D) show the relative positions of clusters C5, C9 and C12 cells. D: Merged image of pictures obtained with red (receptor signal) and blue (DAPI labeled in nuclei) filters.