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Table 1 Hemocytes are attracted to the neurogenic niche.

From: Primary Neuronal Precursors in Adult Crayfish Brain: Replenishment from a Non-neuronal Source

1. Dissociated cells from crayfish tissues labeled with CTG. Total number of niches Percent of niches with CTG labeled cells
Green Gland 10 0%
Hepatopancreas 11 9%
Hematopoietic Tissue 12 9%
Hemocytes 30 77%
2. Changes in cell culture medium with CTG labeled hemocytes   
Hemocytes + 5-HT × 10-9 M 12 17%
Hemocytes + MMS × 10-8 M 16 31%
3. Treatment prior to co-culture, culture + CTG labeled hemocytes   
5-day PCPA pretreatment 8 12.5%
Sheath left on dissected brain 12 0%
  1. The relationship between the neurogenic niche and dissociated CellTracker™ Green (CTG)-labeled cells from four crayfish tissues (green gland, hepatopancreas, hematopoietic tissue and hemolymph) was examined in co-cultured, desheathed brains. Complete exposure to the niche/ventral brain surface appears to be essential for the niche region to attract CGT-labeled hemocytes in vitro, because no cells are attracted to the niche when sheathed brains are placed in co-culture with CTG-labeled hemocytes. The number and the percentage of total niches containing CTG+ cells appear in the second and third columns. The Chi-square test of independence was used to test the significance of these findings; the result is chi square (7, N = 111) = 34.8885, p < 0.001, indicating that the number of niches containing CTG+ cells was significantly dependent on the cell type. The attraction of hemocytes to the niche relative to other cell types is therefore of statistical significance.