Primary Neuronal Precursors in Adult Crayfish Brain: Replenishment from a Non-neuronal Source

BMC Neuroscience

Table 1 Hemocytes are attracted to the neurogenic niche.

1. Dissociated cells from crayfish tissues labeled with CTG.	Total number of niches	Percent of niches with CTG labeled cells
Green Gland	10	0%
Hepatopancreas	11	9%
Hematopoietic Tissue	12	9%
Hemocytes	30	77%
2. Changes in cell culture medium with CTG labeled hemocytes
Hemocytes + 5-HT × 10^-9 M	12	17%
Hemocytes + MMS × 10^-8 M	16	31%
3. Treatment prior to co-culture, culture + CTG labeled hemocytes
5-day PCPA pretreatment	8	12.5%
Sheath left on dissected brain	12	0%

The relationship between the neurogenic niche and dissociated CellTracker™ Green (CTG)-labeled cells from four crayfish tissues (green gland, hepatopancreas, hematopoietic tissue and hemolymph) was examined in co-cultured, desheathed brains. Complete exposure to the niche/ventral brain surface appears to be essential for the niche region to attract CGT-labeled hemocytes in vitro, because no cells are attracted to the niche when sheathed brains are placed in co-culture with CTG-labeled hemocytes. The number and the percentage of total niches containing CTG+ cells appear in the second and third columns. The Chi-square test of independence was used to test the significance of these findings; the result is chi square (7, N = 111) = 34.8885, p < 0.001, indicating that the number of niches containing CTG+ cells was significantly dependent on the cell type. The attraction of hemocytes to the niche relative to other cell types is therefore of statistical significance.

ISSN: 1471-2202