Figure 2

Histochemistry of NADPH-d in the HN and DMN. Light photomicrographs showing the injury-induced NADPH-d reactivity in the lesioned side of the HN and DMN at 7 (A, B, C) and 14 (D, E, F) days after PNCI. NADPH-d staining is barely detected in HN and DMN neurons in the contralateral intact side of the nuclei (the right side of each figure). Note the marked increase in NADPH-d reactivity both in terms of staining intensity and number of labeled neurons at 7 days in the HN and DMN after PNCI (A, B, C). Also note that the population of NADPH-d(+) neurons in the HN is reduced at 14 days after PNCI, whereas that in the DMN tends to increase (D, E, F) when compared those at 7 days post PNCI. Some shrunken cells (D, arrows) are found to display intense NADPH-d reactivity. In rats receiving EGCG treatment at high dose (50 mg/kg, C, F), the diaphorase reactivity and the number of positive neurons are noticeably reduced in relation to those of non-treated rats (A, D) or animals treated with lower dose EGCG (10 mg/kg, B, E). The ellipse and polygonal dotted line in A outlines the area of the HN and DMN, respectively. V, fourth ventricle; Scale bar = 100 μm.