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Table 1 Response of Myzus persicae clones 4106A (susceptible), 5191A (moderately resistant) and FRC (highly resistant) to imidacloprid and thiamethoxam using two bioassay methods, with and without pretreatment with piperonyl butoxide (PBO)

From: Mutation of a nicotinic acetylcholine receptor β subunit is associated with resistance to neonicotinoid insecticides in the aphid Myzus persicae

   Micro-application Spray application
Treatment Clone LC50 1(mg l-1) 95%CL RF2 LC50 (mg l-1) 95% CL RF2
Imidacloprid 4106A 0.99 0.9-1.4   0.14 0.11-0.17 -
  5191A 31.1 7-69.7 31.4 - - -
  FRC 28% at 1000 mg l-1 n/c n/c 235.02 142.33-476.29 1679
Imidacloprid+PBO 4106A 0.1 0.07-0.15   0.03 0.01-0.10 -
  5191A 1.55 0.56-2.55 14.9 - - -
  FRC 30% at 1000 mg l-1 n/c n/c 7.03 1.77-37.91 234
Thiamethoxam 4106A 0.64 0.54-0.79   0.48 0.42-0.55 -
  5191A 19.7 14.87-25.74 30.8 - - -
  FRC 10% at 1000 mg l-1 n/c n/c 107.84 69.52-185.36 225
Thiamethoxam+PBO 4106A 0.27 0.18-0.32   0.19 0.09-0.41 -
  5191A 1.06 0.77-1.45 3.93 - - -
  FRC 216 110.25-483.48 800 4.86 2.03-14.91 26
  1. 5191A was only tested by micro-application of insecticide.
  2. 1Calculated concentration to kill 50% of individuals. If unobtainable, % mortality at the highest concentration applied
  3. 2 Resistance factor (LC50 resistant/LC50 susceptible). n/c = not calculable
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