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Table 1 Response of Myzus persicae clones 4106A (susceptible), 5191A (moderately resistant) and FRC (highly resistant) to imidacloprid and thiamethoxam using two bioassay methods, with and without pretreatment with piperonyl butoxide (PBO)

From: Mutation of a nicotinic acetylcholine receptor β subunit is associated with resistance to neonicotinoid insecticides in the aphid Myzus persicae

  

Micro-application

Spray application

Treatment

Clone

LC50 1(mg l-1)

95%CL

RF2

LC50 (mg l-1)

95% CL

RF2

Imidacloprid

4106A

0.99

0.9-1.4

 

0.14

0.11-0.17

-

 

5191A

31.1

7-69.7

31.4

-

-

-

 

FRC

28% at 1000 mg l-1

n/c

n/c

235.02

142.33-476.29

1679

Imidacloprid+PBO

4106A

0.1

0.07-0.15

 

0.03

0.01-0.10

-

 

5191A

1.55

0.56-2.55

14.9

-

-

-

 

FRC

30% at 1000 mg l-1

n/c

n/c

7.03

1.77-37.91

234

Thiamethoxam

4106A

0.64

0.54-0.79

 

0.48

0.42-0.55

-

 

5191A

19.7

14.87-25.74

30.8

-

-

-

 

FRC

10% at 1000 mg l-1

n/c

n/c

107.84

69.52-185.36

225

Thiamethoxam+PBO

4106A

0.27

0.18-0.32

 

0.19

0.09-0.41

-

 

5191A

1.06

0.77-1.45

3.93

-

-

-

 

FRC

216

110.25-483.48

800

4.86

2.03-14.91

26

  1. 5191A was only tested by micro-application of insecticide.
  2. 1Calculated concentration to kill 50% of individuals. If unobtainable, % mortality at the highest concentration applied
  3. 2 Resistance factor (LC50 resistant/LC50 susceptible). n/c = not calculable
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BMC Neuroscience

ISSN: 1471-2202

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