The human D4ECR1 exhibits transcriptional activity in dissociated cultures of neonate rat frontal cortex. D4ECR1 (1 μg) construct was transfected into dissociated cultures of frontal cortex obtained from 2 and 5 days old Wistar rats under basal conditions. The transcriptional activity of the D4ECR1 is different from pGL3p alone. This effect was found to be significant (Student's T-test, p < 0.001, ***). Values obtained in three independent experiments per triplicate (n = 9).