Figure 4

Lgi1 expression at embryonic stage E13.5. A Sagittal view (anti-GFP-peroxidase stained) of the whole embryo indicating areas of Lgi1 expression seen in Figures A.1 - A.4. A.1 Lgi1 expression is seen in cells in the periventricular zone (pvz), ganglionic eminence (ge) and septum neuroepithelium (sne). Lgi1 is also expressed in A.2 the medulla oblongata (mo) and A.3 the choroid plexus adjacent to the 4^th ventricle (4thv). A.4 In the oral cavity, Lgi1 is expressed in the tongue (tg). B Frontal section (H & E staining) indicating the area seen in Figure B.1. B.1 Frontal section showing Lgi1 expression throughout the tongue (tg), mandibular process and lower molar region (pm). Scale bars = 100 μm.