Figure 3

Lgi1 expression at embryonic stages E11.5 and E12.5. A Coronal section (H & E staining) identifies regions seen in Figures A.1 and A.2. In A.1, Lgi1 expression was seen in the trigeminal ganglion (tg), optic stalk (os), and optic cup (op). B Sagittal view of the E12.5 mouse whole embryo identifying regions seen in Figures B.1 and B.2. In B.1, a midsagittal section (anti-GFP-peroxidase stained) of the cephalic region shows Lgi1 expression in the choroid plexus (chp), inferior colliculi (ic), and cerebellar plate (cbp). In B.2, a midsagittal section at the cephalic region of the embryo showing Lgi1 expression in the pretectum (pt), ventral tegmental area (vt) and midbrain (mb). Scale bars = 100 μm (B.1, B.2), 50 μm (A.1, A.2).