Figure 13

Laser scanning confocal images showing co-expression of Lgi1 and beta-III tubulin in the cortical plate and ventricular zone of mouse brain at embryonic stage E14.5. A. Sagittal view (anti-GFP-peroxidase stained) of whole embryo indicating regions shown in Figures A.1-7. A.1. Shows Lgi1 expressing cells through the ventricular zone (vz) and cortical plate (cp). A.2. Shows beta-III tubulin expression in the cortical plate, overlaid in A.3 and shown in higher magnification in A.4 and A.5 respectively and contrasted in DAPI in A.6. A.7. Shows Lgi1 expressing cells overlaid with beta-III tubulin in the marginal zone (mz), cortical plate (cp), and ventricular zone (vz). Beta-III tubulin is not expressed in intermediate zone (iz). Lgi1 signal is seen in intermediate (iz) and subventricular (sbv) zones. Lateral ventricle (lv). Scale bars = 20 μm.