Flowchart of the EEG acquisition and FUS sonication. Block-A represents the baseline period. The baseline EEG was recorded for ten minutes after the EEG signals stabilized following the administration of anesthesia. Block-B (named as 'Pre-FUS') indicates the time-interval after observing significant evidence of ictal behavior (e.g., bilateral forepaw-twitches) and just before the first sonication. Block-C represents the three-minute period of the first sonication (named as 'FUS1'), and Block-E represents the second three-minute sonication interval (named as 'FUS2'). Block-D represents the time-interval after the first sonication (named as 'Post1'), and Block-F represents the time-interval after the second sonication (named as 'Post2').